TruePlay is a balance of the speakers, in response to perceived acoustics, and the ‘nominal’ response programmed by Sonos.

AirPlay 2 and Spotify(LAN) are a method by which data is sent to the speakers.

They aren’t the same kind of thing. It’s like asking ‘do Volkswagen Beetles taste the same as Oranges?’ in my mind, so I’m guessing I don’t understand your question. 

Thanks for you reply, let me try again.

I believe that I hear differences between something played from Spotify between using direct playback - or playback through the Sonos App - and connecting via Airplay 2 from the Spotify app. 

I am wondering if the TruePlay response is applied via both playback methods (Sonos app accessing Spotify account) vs using an Airplay2 connection via the Sonos app.

Gotcha. 

TruePlay is applied to the speakers.  No matter what signal is sent to them, it will have TruePlay applied.

The signal you send to the speakers (or the speakers get on their own) is possibly different. Spotify has told Sonos’ speakers to get data from server X. Spotify (and most streamers) supply the signal from their mobile apps from server Y. It’s feasible that this data is the same, it is equally feasible that the file (size, bitrate, depth, etc) are different. And then you need to add possible compression by AirPlay 2 (or Bluetooth, if you were using that) into the mix. Essentially, the ‘file’ being played by the Sonos may not be the same. 

Many thanks!