Yes, you can group all of the speakers you listed together in the Sonos app to play the same music in sync.

Presumably you have the stereo-paired Fives and Sub in “Room1” and the paired Ones in “Room2”? Use the icon at bottom right beside the volume slider on the home page of one of the rooms. Then group Room1 and Room2. If you have different sources playing in each room you can also select which of them you want playing to both rooms. 
 

When you add a third room an “Everywhere” button will appear, and you can also create commonly-used groupings to select, so get out and buy more speakers!